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HINT1 Rabbit pAb

Anti-HINT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220596

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220596
Product Name:   Anti-HINT1 Rabbit Polyclonal Antibody
Synonyms:   HINT; NMAN; PKCI-1; PRKCNH1
UNIPROT ID:   P49773 (Gene Accession – NP_005331 )
Background:    The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene.
Immunogen:    Synthetic peptide of human HINT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gasrtic cancer tissue using 220596(HINT1 Antibody) at a dilution of 1/50(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gasrtic cancer tissue is first treated with the synthetic peptide and then with 220596(Anti-HINT1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220596(Anti-HINT1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261741(Anti-HINT1 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: LoVo cells;
Primary antibody: 220596(HINT1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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