Cat.#: N263365 |
Product Name: Anti-Histone H2A.X Rabbit Monoclonal Antibody |
Synonyms: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X |
UNIPROT ID: P16104 |
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
Immunogen: A synthetic peptide of human Histone H2A.X |
Applications: WB,IHC-F,IHC-P,ICC/IF,IP |
Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
Host Species: Rabbit |
Clonality: Rabbit Monoclonal |
Clone ID: R02-3C8 |
MW: Calculated MW: 15 kDa; Observed MW: 15 kDa |
Isotype: IgG |
Purification: Affinity Purified |
Species Reactivity: Human |
Conjugation: Unconjugated |
Modification: Unmodified |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Research Areas: EpigeneticsyyyyyHistone methylation |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Histone H2A.X antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | Immunocytochemistry analysis of Histone H2A.X (green) in Hela using Histone H2A.X antibody,and DAPI(blue). | Western blot analysis of Histone H2A.X in Hela, Ramos lysates using Histone H2A.X antibody. | |
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