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Histone H2A.X Rabbit mAb

Anti-Histone H2A.X Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N263365

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N263365
Product Name:   Anti-Histone H2A.X Rabbit Monoclonal Antibody
Synonyms:   H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
UNIPROT ID:   P16104
Background:    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    A synthetic peptide of human Histone H2A.X
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R02-3C8
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone methylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Histone H2A.X antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Histone H2A.X (green) in Hela using Histone H2A.X antibody,and DAPI(blue).

Western blot analysis of Histone H2A.X in Hela, Ramos lysates using Histone H2A.X antibody.
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