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HMGB3 Rabbit pAb

Anti-HMGB3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219853

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219853
Product Name:   Anti-HMGB3 Rabbit Polyclonal Antibody
Synonyms:   HMG4, HMG-4, HMG2A, HMG-2a
UNIPROT ID:   O15347 (Gene Accession – NP_005333 )
Background:    High-mobility group protein B3 is a protein that in humans is encoded by the HMGB3 gene. HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 and HMG2, HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription.
Immunogen:    Synthetic peptide of human HMGB3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 219853(HMGB3 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 219853(Anti-HMGB3 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane 1-2: Mouse lung tissue, Mouse brain tissue;
Primary antibody: 219853(HMGB3 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1.5 minutes
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