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NCLN Rabbit pAb

Anti-NCLN Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218753

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218753
Product Name:   Anti-NCLN Rabbit Polyclonal Antibody
Synonyms:   NET59
UNIPROT ID:   Q969V3 (Gene Accession – BC013283 )
Background:    May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
Immunogen:    Fusion protein of human NCLN
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 218753(NCLN Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 218753(Anti-NCLN Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and A172 cell lysates;
Primary antibody: 218753(NCLN Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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