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NCR1 Rabbit pAb

Anti-NCR1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220435

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220435
Product Name:   Anti-NCR1 Rabbit Polyclonal Antibody
Synonyms:   LY94; CD335; NKP46; NK-p46
UNIPROT ID:   O76036 (Gene Accession – NP_001138929 )
Background:    Natural killer (NK) cells mediate MHC nonrestricted cytotoxicity. There are many candidate molecules for NK cell recognition structures, including inhibiting and activating receptors. Using a monoclonal antibody capable of triggering NK-mediated lysis, Sivori et al. (1997) identified a cell surface molecule of 46 kD that they termed p46. The molecule lacks N-linked sugars and is expressed on resting and activated NK cells but not on T cells or B cells. In addition to lytic activity, monoclonal antibody-mediated crosslinking induces intracellular calcium increases and cytokine production.
Immunogen:    Synthetic peptide of human NCR1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220435(NCR1 Antibody) at a dilution of 1/50(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220435(Anti-NCR1 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse lung tissue lysate;
Primary antibody: 220435(NCR1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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