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NHP2 Rabbit pAb

Anti-NHP2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218973

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218973
Product Name:   Anti-NHP2 Rabbit Polyclonal Antibody
Synonyms:   DKCB2; NHP2P; NOLA2
UNIPROT ID:   Q9NX24 (Gene Accession – BC000009 )
Background:    This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human NHP2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 218973(NHP2 Antibody) at a dilution of 1/55(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 218973(Anti-NHP2 Antibody) at dilution 1/55.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 218973(Anti-NHP2 Antibody) at a dilution of 1/55.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D225589(Anti-NHP2 Antibody) at dilution 1/55.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human spleen tissue lysate;
Primary antibody: 218973(NHP2 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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