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p21 Rabbit pAb

Anti-p21 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225003

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225003
Product Name:   Anti-p21 Rabbit pAb
Synonyms:   CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
UNIPROT ID:   P38936
Background:    The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.
Immunogen:    The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160
Applications:    ICC/IF,WB,IHC-F,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 18 kDa; Observed MW: 26 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of p21 in rat lung tissue using p21 antibody(red),and DAPI (blue).

Immunofluorescence analysis of p21 in mouse kidney tissue using p21 antibody(red),and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded rat lung tissue using p21 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only.

Immunohistochemistry analysis of paraffin-embedded mouse liver tissue using p21 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only.

Western blot analysis of p21 in various lysates using p21 antibody
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