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Phospho-Raf1 (Ser259) Rabbit mAb

Anti-Phospho-Raf1 (Ser259) Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N262806

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N262806
Product Name:   Anti-Phospho-Raf1 (Ser259) Rabbit Monoclonal Antibody
Synonyms:   RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
UNIPROT ID:   P04049
Background:    Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser259 of human Raf1
Applications:    WB,IHC-P
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R07-4J4
MW:    Calculated MW: 73 kDa; Observed MW: 73 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Raf1 (Phospho-Ser259) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Phospho-Raf1 (Ser259) in K562, C6, 3T3, Hela lysates using Phospho-Raf1 (Ser259) antibody.
          Publications