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POLD1 Rabbit pAb

Anti-POLD1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213103

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213103
Product Name:   Anti-POLD1 Rabbit Polyclonal Antibody
Synonyms:   CDC2; MDPL; POLD; CRCS10
UNIPROT ID:   P28340 (Gene Accession – BC008800 )
Background:    This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3′ to 5′ exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]
Immunogen:    Fusion protein of human POLD1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 213103(POLD1 Antibody) at a dilution of 1/160(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 213103(Anti-POLD1 Antibody) at dilution 1/160.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: K562 cell lysate;
Primary antibody: 213103(POLD1 Antibody) at dilution 1/1100;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
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