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POU2F1 Rabbit pAb

Anti-POU2F1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216694

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216694
Product Name:   Anti-POU2F1 Rabbit Polyclonal Antibody
Synonyms:   OCT1, OTF1, oct-1B
UNIPROT ID:   P14859 (Gene Accession – BC001664 )
Background:    The OCT1 transcription factor was among the first identified members of the POU transcription factor family. Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.
Immunogen:    Fusion protein of human POU2F1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216694(POU2F1 Antibody) at a dilution of 1/30(cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216694(Anti-POU2F1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 216694(POU2F1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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