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RAB35 Rabbit pAb

Anti-RAB35 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219628

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219628
Product Name:   Anti-RAB35 Rabbit Polyclonal Antibody
Synonyms:   RAY; H-ray; RAB1C
UNIPROT ID:   Q15286 (Gene Accession – NP_006852 )
Background:    Enables GTPase activity; guanyl ribonucleotide binding activity; and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including endosomal transport; plasma membrane to endosome transport; and protein localization to endosome. Located in several cellular components, including clathrin-coated endocytic vesicle; clathrin-coated pit; and intercellular bridge.
Immunogen:    Synthetic peptide of human RAB35
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219628(RAB35 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219628(Anti-RAB35 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 219628(Anti-RAB35 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D260039(Anti-RAB35 Antibody) at dilution 1/40.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse brain tissue, 293T, NIH/3T3 cell lysates;
Primary antibody: 219628(RAB35 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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