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RFWD3 Rabbit pAb

Anti-RFWD3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225401

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225401
Product Name:   Anti-RFWD3 Rabbit pAb
Synonyms:   FANCW; RNF201
UNIPROT ID:   Q6PCD5
Background:    E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098).
Immunogen:    The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423
Applications:    WB,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 85 kDa; Observed MW: 85 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:   
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of RFWD3 in HepG2 lysates using RFWD3 antibody.

Western blot analysis of RFWD3 in 823 lysates using RFWD3 antibody.

Western blot analysis of RFWD3 in MCF-7 lysates using RFWD3 antibody. The lane on the right is blocked with the synthesized peptide.
          Publications