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RPN1 Rabbit pAb

Anti-RPN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217087

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217087
Product Name:   Anti-RPN1 Rabbit Polyclonal Antibody
Synonyms:   OST1; RBPH1
UNIPROT ID:   P04843 (Gene Accession – BC010839 )
Background:    This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Immunogen:    Fusion protein of human RPN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217087(RPN1 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217087(Anti-RPN1 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217087(Anti-RPN1 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D221767(Anti-RPN1 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, A549 cells, MCF7 cells, human liver cancer tissue, Human lung cancer tissue;
Primary antibody: 217087(RPN1 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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