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SRC Rabbit pAb

Anti-SRC Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216805

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216805
Product Name:   Anti-SRC Rabbit Polyclonal Antibody
Synonyms:   ASV; SRC1; c-SRC; p60-Src
UNIPROT ID:   P12931 (Gene Accession – BC011566 )
Background:    This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Immunogen:    Fusion protein of human SRC
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lymphoma tissue using 216805(SRC Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lymphoma tissue is first treated with the fusion protein and then with 216805(Anti-SRC Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216805(Anti-SRC Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D221267(Anti-SRC Antibody) at dilution 1/20.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: PC3 cells;
Primary antibody: 216805(SRC Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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