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TERT Rabbit pAb

Anti-TERT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219857

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219857
Product Name:   Anti-TERT Rabbit Polyclonal Antibody
Synonyms:   TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
UNIPROT ID:   O14746 (Gene Accession – NP_937983 )
Background:    Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
Immunogen:    Synthetic peptide of human TERT
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling,Cancer,Stem Cells,Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219857(TERT Antibody) at a dilution of 1/70(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 219857(Anti-TERT Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 219857(Anti-TERT Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D260502(Anti-TERT Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Human mucinous type of soft tissue sarcoma tissue lysate;
Primary antibody: 219857(TERT Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 90 seconds
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