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TRIM32 Rabbit pAb

Anti-TRIM32 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216859

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216859
Product Name:   Anti-TRIM32 Rabbit Polyclonal Antibody
Synonyms:   HT2A; BBS11; TATIP; LGMD2H
UNIPROT ID:   Q13049 (Gene Accession – BC003154 )
Background:    The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
Immunogen:    Fusion protein of human TRIM32
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 15-50;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216859(TRIM32 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216859(Anti-TRIM32 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216859(Anti-TRIM32 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D221369(Anti-TRIM32 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Lovo cells;
Primary antibody: 216859(TRIM32 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 minutes
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