Email: info@neweastbio.com   |  Call: 6109452007

UBA1 Rabbit pAb

Anti-UBA1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221072

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221072
Product Name:   Anti-UBA1 Rabbit Polyclonal Antibody
Synonyms:   A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
UNIPROT ID:   P22314 (Gene Accession – NP_695012 )
Background:    The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Immunogen:    Synthetic peptide of human UBA1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 221072(UBA1 Antibody) at a dilution of 1/40(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 221072(Anti-UBA1 Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Human fetal brain tissue, 231 cells, 293T cells, Hela cells;
Primary antibody: 221072(UBA1 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
          Publications