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UBE2J2 Rabbit pAb

Anti-UBE2J2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218112

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218112
Product Name:   Anti-UBE2J2 Rabbit Polyclonal Antibody
Synonyms:   UBC6; NCUBE2; NCUBE-2; PRO2121
UNIPROT ID:   Q8N2K1 (Gene Accession – BC113430 )
Background:    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Immunogen:    Fusion protein of human UBE2J2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218112(UBE2J2 Antibody) at a dilution of 1/80(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218112(Anti-UBE2J2 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 218112(Anti-UBE2J2 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D223744(Anti-UBE2J2 Antibody) at dilution 1/80.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2, 293T cell lysates;
Primary antibody: 218112(UBE2J2 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 6 seconds
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