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UBE2N Rabbit pAb

Anti-UBE2N Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215332

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215332
Product Name:   Anti-UBE2N Rabbit Polyclonal Antibody
Synonyms:   UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
UNIPROT ID:   P61088 (Gene Accession – NP_003339 )
Background:    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Immunogen:    Synthetic peptide of human UBE2N
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 215332(UBE2N Antibody) at a dilution of 1/55(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 215332(Anti-UBE2N Antibody) at dilution 1/55.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 215332(Anti-UBE2N Antibody) at a dilution of 1/55.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D163219(Anti-UBE2N Antibody) at dilution 1/55.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse brain tissue, Hela cell, LNCAP cell, HepG2 cell, Jurkat cell lysates;
Primary antibody: 215332(UBE2N Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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