Email: info@neweastbio.com   |  Call: 6109452007

ZAP70 (4B2) Mouse mAb

Anti-ZAP70 (4B2) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261113

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261113
Product Name:   Anti-ZAP70 (4B2) Mouse Monoclonal Antibody
Synonyms:   ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase
UNIPROT ID:   P43403
Background:    The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck.
Immunogen:    Purified recombinant human ZAP-70 protein fragments expressed in E.coli.
Applications:    WB,IP
Recommended Dilutions:    WB: 1/500-1/1000 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4B2-C1-H3
MW:    Calculated MW: 70 kDa; Observed MW: 70 kDa
Isotype:    IgG2b
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation analysis of ZAP70 (4B2) in Jurkat lysates (ZAP70 positive expression cell line)and Ramos lysates(ZAP70 negative expression cell line) using ZAP70 antibody.

Western blot analysis of ZAP70 in CEM and Jurkat lysates, negative in the Ramos lysates using ZAP70 antibody.
          Publications