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ZIC3 ? Rabbit pAb

Anti-ZIC3 ? Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213327

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213327
Product Name:   Anti-ZIC3 Rabbit Polyclonal Antibody
Synonyms:   HTX; HTX1; ZNF203; VACTERLX
UNIPROT ID:   O60481 (Gene Accession – NP_003404 )
Background:    This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.
Immunogen:    Synthetic peptide of human ZIC3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 213327(ZIC3 Antibody) at a dilution of 1/100(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 213327(Anti-ZIC3 Antibody) at dilution 1/100.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cells;
Primary antibody: 213327(ZIC3 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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