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ZKSCAN3 Rabbit pAb

Anti-ZKSCAN3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218231

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218231
Product Name:   Anti-ZKSCAN3 Rabbit Polyclonal Antibody
Synonyms:   ZF47; Zfp47; ZFP306; ZNF306; ZNF309; zfp-47; ZSCAN13; ZSCAN35; dJ874C20.1; dJ874C20.1
UNIPROT ID:   Q9BRR0 (Gene Accession – BC006118 )
Background:    Transcriptional factor that binds to the consensus sequence 5′-[GT][AG][AGT]GGGG-3′ and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.
Immunogen:    Fusion protein of human ZKSCAN3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218231(ZKSCAN3 Antibody) at a dilution of 1/90(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218231(Anti-ZKSCAN3 Antibody) at dilution 1/90.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 218231(Anti-ZKSCAN3 Antibody) at a dilution of 1/90.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D223990(Anti-ZKSCAN3 Antibody) at dilution 1/90.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cell, Mouse spleen tissue lysates;
Primary antibody: 218231(ZKSCAN3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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