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ZNF281 Rabbit pAb

Anti-ZNF281 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218226

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218226
Product Name:   Anti-ZNF281 Rabbit Polyclonal Antibody
Synonyms:   ZBP-99; ZNP-99
UNIPROT ID:   Q9Y2X9 (Gene Accession – BC060820 )
Background:    Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF281, also known as GC-box-binding zinc finger protein 1, ZBP-99 or ZNP-99 (zinc finger DNA-binding protein 99), is a zinc finger protein that belongs to the Krppel C2H2-type zinc finger protein family. It is expressed ubiquitously at low levels with predominant expression in kidney, liver, lymphocytes and placenta. ZNF281 localizes to the nucleus and contains four C2H2-type zinc fingers. ZNF281 plays a role in repressing the transcription of a variety of genes including Gastrin and ODC (ornithine decarboxylase). In particular, ZNF281 functions by binding to the G-rich box in the enhancer region of the gene. Upon DNA damage, ZNF281 may become phosphorylated by Atm or ATR.
Immunogen:    Fusion protein of human ZNF281
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Stem Cells, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218226(ZNF281 Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218226(Anti-ZNF281 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 218226(Anti-ZNF281 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D223984(Anti-ZNF281 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: A549 cell;
Primary antibody: 218226(ZNF281 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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