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ZNF85 Rabbit pAb

Anti-ZNF85 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S215938

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S215938
Product Name:   Anti-ZNF85 Rabbit Polyclonal Antibody
Synonyms:   HPF4; HTF1
UNIPROT ID:   Q03923 (Gene Accession – NP_003420 )
Background:    Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF85 (Zinc finger protein 85), also known as zinc finger protein HPF4 or HTF1, is a member of the ZNF91 family and is thought to be involved in transcriptional regulation. ZNF85 is highly expressed in testicular tissue and localizes to the nucleus. ZNF85 contains sixteen C2H2-type zinc fingers and one KRAB domain through which it is thought to be involved in DNA-binding and transcriptional regulation.
Immunogen:    Synthetic peptide of human ZNF85
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 215938(ZNF85 Antibody) at a dilution of 1/35(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 215938(Anti-ZNF85 Antibody) at dilution 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Raji and PC3 cell lysates;
Primary antibody: 215938(ZNF85 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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