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Arl3-GTP

Anti-Arl3-GTP Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26925

   Size:   30 μl

In Stock

          Product Description          
Anti-Arl3GTP Mouse Monoclonal Antibody
Cat. #:  26925
Gene Symbol:  ARL3
Description:   Anti-Arl3-GTP Mouse Monoclonal Antibody
Background:   Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery.
Immunogen:   Recombinant Arl3 protein (active form)
Applications:   IP, IHC and IF (Not applicable for WB since SDS denatures Arl3 GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Arl3 antibody recognizes active Arl3-GTP from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #10152) were immunoprecipitated with the anti-active Arl3 monoclonal antibody (Cat. #26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl3 monoclonal antibody (Cat. #21070). Input controls are shown in the bottom panel.
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