$495.00
| Anti-Arl3GTP Mouse Monoclonal Antibody |
| Cat. #: 26925 |
| Gene Symbol: ARL3 |
| Description: Anti-Arl3-GTP Mouse Monoclonal Antibody |
| Background: Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery. |
| Immunogen: Recombinant Arl3 protein (active form) |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Arl3 GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Arl3 antibody recognizes active Arl3-GTP from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Arl3 Activation Assay.
Purified His-tagged Arl3 proteins (Cat. #10152) were immunoprecipitated with the anti-active Arl3 monoclonal antibody (Cat. #26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl3 monoclonal antibody (Cat. #21070). Input controls are shown in the bottom panel. |
| 01. Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation J Biol Chem. 2017 PMID: 28848045 |
