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Human C5AR1 Full Length Protein

Human C5AR1 Full Length Protein-Synthetic Nanodisc


Cat.#:  11067

   Size:   50 μg

3-4 weeks

          Product Description          
Cat.#:     11067
Product Name:   Human C5AR1 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   C5A; C5AR; C5R1; CD88
Target:    C5AR1
UNIPROT ID:    P21730
Description:    Human C5AR1 full length protein-synthetic nanodisc
Background:     Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production
Species/Host:    HEK293
Molecular Weight:    The human full length C5AR1 protein has a MW of 39.3 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag C5AR1-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with C5AR1-Nanodisc is 4.088ng/ml.

Figure2. Human C5AR1-Nanodisc, Flag Tag on SDS-PAGE