Subtotal:
$347.00
$445.00
| Cat.#: 28395 | ||||
| Product Name: Anti-CD33(DM77) Rabbit Monoclonal Antibody | ||||
| Synonyms: CD33;SIGLEC3;gp67 | ||||
| Description: Anti-CD33 antibody(DM77) Rabbit Monoclonal Antibody | ||||
| Background: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K. | ||||
| Applications: ELISA; Flow Cyt | ||||
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 | ||||
| Host Species: Rabbit | ||||
| Isotype: Rabbit IgG | ||||
| Purification: Purified from cell culture supernatant by affinity chromatography | ||||
| Species Reactivity: Human CD33 | ||||
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. | ||||
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). | ||||
|
