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Human CGRPR-RAMP1 Full Length Protein

Human CGRPR-RAMP1 Full Length Protein-Synthetic Nanodisc


Cat.#:  11126

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11126
Product Name:   Human CGRPR-RAMP1 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CALCRL; CRLR; LMPHM8
Target:    CGRPR
UNIPROT ID:    Q16602
Description:    Human CGRPR-RAMP1 full length protein-synthetic nanodisc
Background:     The CGRP receptor (CGRPR) is a member of family B G protein coupled receptors (GPCRs), is expressed throughout the trigeminal system, including neurons and endothelial cells. They usually function with accessory proteins such as receptor activity modifying proteins (RAMPs) and Na/H exchange regulatory factors (NHERFs). CGRPR is a heterodimer complex of the calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1). Therapeutics for migraine treatment are mostly targeting CRLR-RAMP1 protein-protein interaction surfaces, thereby blocking CGRP activity.
Species/Host:    HEK293
Molecular Weight:    The human full length CGRPR protein has a MW of 53.0 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CGRPR-RAMP1-Nanodisc (0.2 µg/per well). Serial diluted anti-CGRPR monoclonal antibody (28157) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CGRPR monoclonal antibody binding with CGRPR-RAMP1-Nanodisc is 1216ng/ml.

Figure2. Human CGRPR-RAMP1-Nanodisc, Flag Tag on SDS-PAGE