NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26299 |
Gene Symbol: Beta-catenin, CTNNB, Catenin beta-1 |
Description: Anti-CTNNB1(G34V) Mouse Monoclonal Antibody |
Background: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherent junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutant CTNNB1 (ß-catenin) has been implicated in the pathogenesis of several cancers including melanoma, colorectal cancer, hepatocelluar carcinoma, and ovarian cancer. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers. |
Immunogen: A synthetic peptide from the internal region of CTNNB1 which includes the mutation of G34V, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 0.5 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes G34V mutant, but not wild type CTNNB1 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant CTNNB1 (G34V) and wild type proteins. Purified His-tagged CTNNB1 (G34V) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-CTNNB1(G34V) monoclonal antibody (Cat. #26299). Immunofluorescence: Immunofluorescence of cells expressing CTNNB1 proteins with Anti-CTNNB1(G34V) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (G34V) plasmid, pCDNA3-GFP-CTNNB1 (WT) plasmid or pCDNA3-GFP-CTNNB1 (G34E) plasmid, then fixed and stained with Anti-CTNNB1(G34V) monoclonal antibody (Cat. #26299). |