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Human CXCR2 Full Length Protein

Human CXCR2 Full Length Protein-Synthetic Nanodisc


Cat.#:  11047

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11047
Product Name:   Human CXCR2 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB
Target:    CXCR2
UNIPROT ID:    P25025
Description:    Human CXCR2 full length protein-synthetic nanodisc
Background:     The protein is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36.
Species/Host:    HEK293
Molecular Weight:    The human full length CXCR2 protein has a MW of 40.8 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag CXCR2-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CXCR2-Nanodisc is 2.474ng/ml.

Figure2. Human CXCR2-Nanodisc, Flag Tag on SDS-PAGE