Email: info@neweastbio.com   |  Call: 6109452007

EGFR(D761Y)

Anti-EGFR(D761Y) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26120

   Size:   100 μl

In Stock

          Product Description          

EGFR(D761Y) 

Cat. #:   26120
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(D761Y) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of D761Y, human origin.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(D761Y), but not wild type EGFR from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.

Western blot:  

Western blot analysis of recombinant EGFR(D761Y) and wild type proteins.

Purified His-tagged EGFR(D761Y) protein (amino acids 702-987, lane 2, Cat. # 10240) and corresponding wild type protein (lane 1, Cat. #10210) were blotted with anti EGFR(D761Y) mouse monoclonal antibody (Cat. #26120). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).

Immunofluorescence:  

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR(D761Y) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR(D761Y) plasmid (right column), then fixed and stained with anti EGFR(D761Y) monoclonal antibody (Cat. #26120).

          Related Products          
          Publications