| Cat.#: 28474 |
| Product Name: Anti-EGFR(DM129) Rabbit Monoclonal Antibody |
| Synonyms: EGFR;ERBB;ERBB1;HER1;PIG61;mENA |
| Description: Anti-EGFR antibody(DM129) Rabbit Monoclonal Antibody |
| Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human EGFR |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human EGFR protein, His tagged protein 11218 can bind Rabbit anti-EGFR monoclonal antibody (clone: DM129) in a linear range of 0. 1-18 ng/ml. | 
Figure 2. EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (DM129) on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram) |
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