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EGFR(T854A)

Anti-EGFR(T854A) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26481

   Size:   100 μl

In Stock

          Product Description          

EGFR(T854A)

Cat. #:  26481
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(T854A) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of T854A, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:200
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR (T854A), but not wild type EGFR from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant EGFR (T854A) and wild type proteins.

Purified His-tagged EGFR (T854A) protein (lane 1) and corresponding wild type protein (amino acids 702-987, lane 2) were blotted with anti EGFR(T854A) mouse monoclonal antibody (Cat. #26481).

Immunofluorescence:  

image.png

Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (T854A) antibody.

HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(T854A) plasmid (right column), then fixed and stained with anti-EGFR (T854A) monoclonal antibody (Cat. #26481).

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