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EPCAM (DM147) Rabbit mAb

Anti-EPCAM (DM147) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28491

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28491
Product Name:   Anti-EPCAM(DM147) Rabbit Monoclonal Antibody
Synonyms:   EPCAM;TACSTD1;TROP1;CD326;DIAR5;EGP2;EGP314;EGP40;ESA;GA733-2;HNPCC8;HNPCC-8;KS1:4;KSA;M4S1;MIC18;MK1
Description:    Anti-EPCAM antibody(DM147) Rabbit Monoclonal Antibody
Background:    This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human EPCAM
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human EPCAM protein, His tagged protein 11191 can bind Rabbit anti-EPCAM monoclonal antibody (clone: DM147) in a linear range of 5-100 ng/ml.

Figure 2. EPCAM protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EPCAM (DM147) on Expi293 cells transfected with human EPCAM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and  Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
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