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ERBB2 mAb

Anti-ERBB2 Mouse Monoclonal Antibody
5/5

$239.00

Cat.#:  26127

   Size:   100 μl

In Stock

          Product Description          
ERBB2 Monoclonal Antibody
Cat. #:  26127
Gene Symbol:  ERBB2/HER2/Neu/c-Neu
Description:   Anti-ERBB2 Mouse Monoclonal Antibody
Background:   The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important criteria in clinic evaluating of the progression of breast cancer.
Immunogen:   A synthetic peptide from the C-terminal region of ERBB2, human origin
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:200
Concentration:    1mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Recognizes Erb-B2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant Erb-B2 protein. Purified His-tagged Erb-B2 protein (Cat. #10208) was blotted with anti-Erb-B2 monoclonal antibody (Cat. #26127). Immunohistochemistry:   Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-Erb-B2 monoclonal antibody (Cat. #26127). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
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