NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
Gα12 Monoclonal Antibody
Cat. #: 26026 |
Gene Symbol: GNA12 |
Description: Anti-Gα12 Mouse Monoclonal Antibody |
Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα12 is a member of the G12/13 subfamily of G proteins. Biochemical and physiological functions of Gα12 are being explored. |
Immunogen: A synthetic peptide from the N-terminal region of human Gα12 protein |
Applications: ELISA, WB, IHC, IP |
Recommended Dilutions: ELISA 1:1000-1:5000 WB 1:100-1:2000 IHC 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Gαq subfamily antibody recognizes all members of the Gq subfamily of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Gα12 proteins. MEF whole-cell lysate was used for western blot with anti- Gα12 mouse monoclonal antibody (cat. # 26026). |
1. Conformational Profiling of the AT1 Angiotensin II Receptor Reflects Biased Agonism, G Protein Coupling, and Cellular ContextJ Biol Chem. 2017 PMID: 28213525 |
2. Gα12 gep oncogene deregulation of p53-responsive microRNAs promotes epithelial-mesenchymal transition of hepatocellular carcinomaOncogene. 2014 PMID: 25065598 |