$495.00
| Anti-Gα13GTP Mouse Monoclonal Antibody |
| Cat. #: 26902 |
| Size: 30 µL |
| Gene Symbol: Gna13 |
| Description: Anti-Gα13-GTP Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
| Immunogen: Recombinant full length active Gα13 protein |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Gα13 GTPase) |
| Published Applications: IF, IHC and IP – Click for Details |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
IP/WB analysis of active Gα13 proteins.
Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004). |
Immunohistochemistry:
 Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells. |
| 1. Elabela-APJ axis attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal ferroptosis Free Radic Biol Med. 2023 PMID: 36681202 |
| 2. The GABA B receptor mediates neuroprotection by coupling to G 13 Sci Signal. 2021 PMID: 34665640 |
| 3. Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor Thromb Res. 2017 PMID: 28609704 |
| 4. Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization J Biol Chem. 2011 PMID: 21771786 |
| 5. Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2 Br J Pharmacol. 2011 PMID: 20958291 |
| 6. Ga12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation Carcinogenesis. 2010 PMID: 20478922 |
| 7. Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency J Biol Chem. 2009 PMID: 19654325 |
