$495.00
| Anti-GαsGTP Mouse Monoclonal Antibody |
| Cat. #: 26906 |
| Size: 30 µL |
| Gene Symbol: Gnas |
| Description: Anti-Gαs-GTP Mouse Monoclonal Antibody |
| Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαs represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases. |
| Immunogen: Recombinant full length protein of active Gαs |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures Gαs GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-active Gαs antibody recognizes active Gαs of vertebrates |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Gαs Activation Assay.
Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (Cat. No. 26906). Immunoblot was with an anti- Gαs monoclonal antibody (Cat. No. 26006). |
