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GITR (DM78) Rabbit mAb

Anti-GITR (DM78) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28397

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28397
Product Name:   Anti-GITR(DM78) Rabbit Monoclonal Antibody
Synonyms:   AITR; GITR; TNFRSF18; CD357
Description:    Anti-GITR antibody(DM78) Rabbit Monoclonal Antibody
Background:    This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation; and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human GITR
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human GITR protein, hFc-His tagged protein 11146 can bind Rabbit anti-GITR monoclonal antibody  (clone: DM78) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human GITR  (B) were surface stained with Rabbit anti-GITR monoclonal antibody 1µg/ml  (clone: DM78) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody  (clone: DM78) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Affinity ranking of different Rabbit anti-GITR mAb clones by titration of different concentration onto PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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