$445.00
| Cat.#: 28287 |
| Product Name: Anti-His Tag(DM1) Rabbit Monoclonal Antibody |
| Synonyms: his tag |
| Description: Anti-His Tag Antibody(DM1) Rabbit Monoclonal Antibody |
| Background: The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1); Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteinsin bacteria; insect cells; and mammalian cells. |
| Applications: ELISA; WB |
| Recommended Dilutions: WB 1:1000 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus. His Tag |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. Western blot analysis of his-tag fusion protein (70kDa, 20ng) using His-Tag antibody (28287) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000 dilution. |
