| Cat.#: 11296 |
| Product Name: Human ACE2 Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: ACE-2;CEH;CE2 |
| Target: ACE2 |
| UNIPROT ID: Q9BYF1 |
| Description: Recombinant Human ACE2 Protein with N-terminal 6xHis tag |
| Background: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 84.4 kDa after removal of the signal peptide. The apparent molecular mass of His-ACE2 is approximately 100-130 kDa due to glycosylation. |
| Molecular Characterization: 6×His ACE2(Gln18-Ser740) |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human ACE2 Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human ACE2, His Tagged proteinï¼11296ï¼can bind S-RBD, hFc tagged protein 11295 in a linear range of 0.128-80.0 ng/ml. |
|
