| Cat.#: 11198 |
| Product Name: Human AFP Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: AFPD;FETA;HPAFP |
| Target: AFP |
| UNIPROT ID: P02771 |
| Description: Recombinant human AFP protein with C-terminal human Fc tag |
| Background: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 92.6 kDa after removal of the signal peptide.The apparent molecular mass of AFP-hFc is approximately 100-130 kDa due to glycosylation. |
| Molecular Characterization: AFP(Arg19-Val609) hFc(Glu99-Ala330) |
| Purity: The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human AFP Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AFP Protein, hFc Tag(11198) can bind Anti-AFP antibody(DMC281), IgG1 Chimeric mAb in a linear range of 0.64-16 ng/mL. |
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