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Arl1(Q71L) Protein

Arl1 Protein Q71L mutant
5/5

$239.00

Cat.#:  10156

   Size:   100 μl

In Stock

            Product Description          
Arl1(Q71L) Protein
Cat. #:  10156
Product Name:  Arl1 Protein Q71L mutant
Synonyms:  ADP-ribosylation factor-like 1, ARFL1
Source:   Human, recombinant full length, His6-tag
Expression Host Species:   E. coli
Molecular Weight:   20 kDa
Purity:   >95% by SDS-PAGE
Introduction:   Arl1 is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform.
Amino Acid Sequence   (1-181, Q71L)
MGGFFSSIFSSLFGTREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVETVTYKNLKFQVWD LGGLTSIRPYWRCYYSNTDAVIYVVDSCDRDRIGISKSELVAMLEEEELRKAILVVFANKQDMEQAM TSSEMANSLGLPALKDRKWQIFKTSATKGTGLDEAMEWLVETLKSRQ
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  1 mg/mL
Storage:   -80°C
Preparation Instructions:   Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arl1 Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining
References:   1. Hong, J.-X. et al., J. Biol. Chem. 273: 15872-15876, 1998. 2. Zhang, G.-F. et al., J. Biol. Chem. 270: 21-24, 1995.
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