Pioneering GTPase and Oncogene Product Development since 2010

Email us: info@neweastbio.com

Call us: 6109452007

Human ALK Protein, hFc Tag

Recombinant Human ALK Protein with C-terminal human Fc tag
5/5

$415.00

Cat.#:  11235

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11235
Product Name:   Human ALK Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD246;NBLST3
Target:    ALK
UNIPROT ID:    Q9UM73
Description:    Recombinant Human ALK Protein with C-terminal human Fc tag
Background:     This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 136.4 kDa after removal of the signal peptide.The apparent molecular mass of ALK-hFc is approximately 130-250 kDa due to glycosylation.
Molecular Characterization:    ALK(Ser31-Gln692) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human ALK Protein, hFc Tag on SDS-PAGE under reducing condition
          Publications