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Human B7-1 Protein, hFc Tag

Recombinant human B7-1 protein with C-terminal human Fc tag
5/5

$555.00

Cat.#:  11283

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11283
Product Name:   Human B7-1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD80;7;7-1;7.1;B1;D28LG;D28LG1;AB7
Target:    B7-1
UNIPROT ID:    P33681
Description:    Recombinant human B7-1 protein with C-terminal human Fc tag
Background:     The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 50.0 kDa after removal of the signal peptide.
Molecular Characterization:    B7-1(Val35-Asn242) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein (11283) can bind Human CTLA4, mFc-His tagged protein 11145 in a linear range of 0.048-2.094 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein (11283) can bind Human CD28, mFc-His tagged protein 11142   in a linear range of 125-4000 ng/ml.

Figure 4. HEK293 cell line transfected with irrelevant protein  (B) and human CD28  (A) were surface stained with Human B7-1 , hFc tagged protein (11283) 1 µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Figure 5. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human B7-1 Protein, hFc Tag (11283) can bind Anti-B7-1 Rabbit mAb in a linear range of 3.2-80 ng/mL.
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