| Cat.#: 11173 |
| Product Name: Human B7-1 Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: CD80;7;7-1;7.1;B1;D28LG;D28LG1;AB7 |
| Target: B7-1 |
| UNIPROT ID: P33681 |
| Description: Recombinant Human B7-1 Protein with C-terminal Mouse Fc and 6xHis tag |
| Background: The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation. |
| Molecular Characterization: B7-1(Val35-Asn242) mFc(Pro99-Lys330) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human B7-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human B7-1, mFc-His tagged protein (11173) can bind anti-B7-1 monoclonal antibody,Rabbit mAb clone: DM111 in a linear range of 0.13-80 ng/ml. | 
Figure 3. Flow cytometry analysis with 15 µg/ml Human B7-1 Protein, mFc-His tag (11173) on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). | |
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