Cat.#: 11177 |
Product Name: Human B7-H4 Protein |
Size : 10 µg, 50 µg and 100 µg |
Synonyms: B7-H4;B7h.5;B7H4;B7S1;B7X;PRO1291;VCTN1 |
Target: B7-H4 |
UNIPROT ID: Q7Z7D3 |
Description: Recombinant Human B7-H4 protein with C-terminal human Fc |
Background: This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. |
Species/Host: HEK293 |
Molecular Weight: The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide.The apparent molecular mass of B7-H4-hFc is approximately 100-130 kDa due to glycosylation. |
Molecular Characterization: B7-H4 (Phe29-Ala258) hFc(Glu99-Ala330) |
Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
 Figure 1. Human B7-H4 Protein, hFc Tag on SDS-PAGE under reducing condition. |  Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human B7-H4, hFc-His tagged protein (11177) can bind Anti-B7-H4 Neutralizing antibody 28081 in a linear range of 3.2-80 ng/ml. |
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