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Human BAFF-R Protein, hFc Tag

Recombinant Human BAFF-R protein with C-terminal human Fc


Cat.#:  11306

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11306
Product Name:   Human BAFF-R Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   BAFFR;NFRSF13C;AFF-R;ROMIX;D268;VID4;rolixin
Target:    BAFF-R
Description:    Recombinant Human BAFF-R protein with C-terminal human Fc
Background:     B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of Baff in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular cysteine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 32.7 kDa after removal of the signal peptide.The apparent molecular mass of BAFF-R-hFc is approximately 35-55 kDa due to glycosylation.
Molecular Characterization:    BAFF-R(Ser7-Ala71) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human BAFF-R Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human BAFFR, hFc tagged protein (11306) can bind Anti-BAFFR Neutralizing antibody 28054 in a linear range of 3.2-80 ng/ml.