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Human CD171 Protein, His Tag

Recombinant Human CD171 Protein with C-terminal 6XHis tag


Cat.#:  11239

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11239
Product Name:   Human CD171 Protein
Size :    10 µg, 50 µg and 100 µg
Target:    CD171
UNIPROT ID:    P32004
Description:    Recombinant Human CD171 Protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 124.0 kDa after removal of the signal peptide.The apparent molecular mass of CD171-His is approximately 130-250 kDa due to glycosylation.
Molecular Characterization:    CD171(Ile20-Glu1120) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD171 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human CD171 Protein, His Tag(11239) can bind Anti-CD171 Rabbit mAb in a linear range of 0.64-16 ng/mL.