| Cat.#: 12232 |
| Product Name: Human CD20 Full Length Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: B1; Bp35; CVID5; FMC7; LEU-16; MS4A2; S7 |
| Target: CD20 |
| UNIPROT ID: P11836 |
| Description: Human CD20 full length protein membrane nanoparticles (MNPs) |
| Background: A member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length CD20 protein has a MW of 33.1 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure1. Elisa plates were pre-coated with 0.5 µg/per well purified human CD20 full length membrane nanoparticles. Serial diluted anti-CD20 monoclonal antibody (28152) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD20 monoclonal antibody binding with CD20 full length membrane nanoparticles is 128.8ng/ml. |    
Figure2. FACS analysis of CD20 MNPs A. Negative Control 1: CD20 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CD20 antibody (28152) at 2µg/ml, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CD20 full length membrane nanoparticles samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2µg/ml, followed by Goat anti-human IgG 488 secondary antibody. D. CD20 full length membrane nanoparticles samples were stained with anti-CD20 antibody (28152) at 2µg/ml, followed by Goat anti-human IgG 488 secondary antibody. |
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